Acute histopathological responses and long-term behavioral ... N2 . Extent and distribution of GLAST and GLT-1 were studied in a rat model of controlled cortical impact injury (CCII). On Day 4 of treatment, a catheter was placed in the femoral artery using isoflurane anaesthesia. 1 , Minnette G. Son M.D. In one experiment, the hemodynamic effects of Epo were determined after controlled cortical impact injury (CCII) by measuring mean arterial pressure, intracranial pressure, CBF using laser Doppler flowmetry . The classical three-point bending and impact tests were carried out for three rat bone groups: control (SHO), the humerus of animals under the . 2001 Feb; 18(2):175-86. Controlled cortical impact injury affects dopaminergic transmission in the rat striatum. A focal cortical impact was delivered at a controlled velocity of 4 m/s, duration of 500 ms, using a 3- or 6-mm tip (PND 7 or 17, respectively). juvenile rats, but few have been developed in the adult rat. Male Wistar rats (n = 65 S-100B, NSE group and n = 55 GFAP group) underwent a severe cortical impact injury (100PSI, 2 mm deformation). Rats given controlled cortical impact injury, a model of closed-head TBI, show altered dopamine neurotransmission (Wagner et al., 2005) and progressive loss of nigrostriatal neurons (Hutson et al . Yet little is known about the expression and activities of genes involved in HA catabolism after TBI. Contusions usually appear near the area of external impact or in regions of the In our rat model of pediatric TBI using controlled cortical impact (CCI), dietary DHA begun one day before injury improved cognitive function and decreased oxidative stress and white matter damage (Schober et al., 2016). Dana D. Dean, Neuroscience Institute, Baylor Scott & White Health, Temple, Texas. It is involved in several pharmacological activities [ 10 ] and cellular signaling [ 11 - 15 ]. Severe controlled cortical impact (CCI) and severe weight drop injury (WDI) were studied for comparison. To study the increased vulnerability of the traumatized brain to secondary insults, bilateral carotid occlusion was produced after a controlled cortical impact injury in rats. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Regional cerebral blood flow (rCBF) and Iaser-Doppler perfusion were measured in rats after controlled cortical impact injury (CCII), a model of traumatic brain injury. Immediately after the impact injury and the NO and microdialy- Controlled cortical impact (CCI) is a mechanical model of traumatic brain injury (TBI) that was developed nearly 30 years ago with the goal of creating a testing platform to determine the biomechanical properties of brain tissue exposed to direct mechanical deformation. Controlled cortical impact: The animal model that may change brain injury Traumatic brain injury (TBI) is one of the leading causes of disability and death around the world. This study compares the acute inflammatory response to TBI over time, as measured by MPO activity, in the WD and controlled cortical impact (CCI) models. 1.2.1 Pneumatic Controlled Cortical Impact . [ 48 , 68 ] The P17 rat brain is developmentally similar to the 2-4 year old human, the pediatric age at greatest risk of poor TBI outcomes, in terms of key indices . pressed CO2 reactivity after mild controlled cortical impact (CCI) injury in rats. Additional information about DHA effects on the inflammatory response of the developing brain to TBI are lacking. Controlled cortical impact injury. Anesthetized adult Sprague-Dawley rats were traumatized using WD (10-g weight dropped 5 cm) or CCI (4 m/sec, 2.5 mm depth). AU - Zhang, Fangyi. Immediately after the impact injury and the NO and microdialysis probes could be replaced, there was an increase from baseline in NO concentration of 83 ± 16 (SE) nM . 1 , Shane M. Sprague A.A. 1 , Farrokh Farrokhi M.D. 1 , Matthew N. Henry M.D. Rats were administered either vehicle or folic acid in an 80 g/kg or 800 g/kg dose. (2009) Severity of controlled cortical impact traumatic brain injury in rats and mice dictates degree of behavioral deficits. BibTeX @ARTICLE{Xu11earlymicrostructural, author = {Su Xu and Jiachen Zhuo and Jennifer Racz and Da Shi and Steven Roys and Gary Fiskum and Rao Gullapalli}, title = {Early microstructural and metabolic changes following controlled cortical impact injury in rat: a magnetic resonance imaging and spectroscopy study}, journal = {J Neurotrauma}, year = {2011}, pages = {2091--2102}} To the best of our knowledge, there is no closed-head model using a commercially available device, including the Controlled Cortical Impact (CCI) device. Control rats received vehicle (0.01% ethanol; ). Namely, subsequent to transient decline in 18-month-old AL rats (* p < 0.05), cortical corticosterone level was significantly increased in 24-month-old rats in comparison to the levels estimated in control, 6-month-old rats (by 2.7 fold; * p < 0.05). The controlled cortical impact (CCI) is a widely-used and well-characterized model which produces clear motor and cognitive deficits that are typically seen after human TBI. Controlled cortical impact surgery After 5 days of acclimatization, rats were submitted to unilateral cortical contusion using the CCI model of TBI. Method. The utility of magnetic resonance imaging (MRI) for the assessment of contusion volume after severe CCI was also established. Chronic methylphenidate treatment enhances striatal dopamine neurotransmission after experimental traumatic brain injury. Following a controlled cortical impact (CCI) on young adult male rats, cortical and hippocampal tissue of the injured hemisphere and matching contralateral material was harvested at early (4, 12, and 24 hours) and extended (3 and 7 days) time points post-procedure. Brain nitric oxide changes after controlled cortical impact injury in rats. Authors Dana D Dean 1 , Joseph A Frank 2 , L Christine Turtzo 3 Affiliations 1 Neuroscience Institute, Baylor Scott & White Health, Temple, Texas . We investigated whether a learning impairment after a controlled cortical impact (CCI) injury was associated with alterations in molecules involved in synaptic plasticity and learning and memory. Controlled Cortical Impact in the Rat Curr Protoc Neurosci. Briefly, the model consists of impacting the exposed dura and underlying cortex after a craniectomy with a cylinder driven by a pneumatic impactor. Reversal of attenuation of cerebrovascular reactivity to hypercapnia by a nitric oxide donor after controlled cortical impact in a rat model of traumatic brain injury Fangyi Zhang M.D. The purpose of this study was to determine the effect of mild or moderate hypoxemia on early histologic and motor functional outcome after controlled cortical impact (CCI) in rats. In this paper, the influence of nesfatin-1 administration on rat humerus mechanical properties was analyzed. JN. Motor function was assessed with beam-balance/beam-walk and rotarod tasks on post-operative days 1-5 and every other day from 1-19, respectively. Methods: Sham procedures or a unilateral parietal controlled cortical impact injury was induced. We tested the correlation between the extra- and intracranial pressures during severe FPI in rat (2.6-2.9 atm), using pressure probes (diameter 0.34 mm) with high frequency (500 Hz) and high pressure range (1-5 atm). Recently, an injury model termed controlled cortical impact injury (CCII), involving direct cortical deformation using a rigid cylinder, was described for ferrets and later for rats . Contusion is a bruise on the brain surface and includes hemorrhage and less severe breakdown of the bloodbrain - barrier. 1 In the presence of L-arginine (10 CCI TBI model. Initially used to model TBIs produced by automotive crashes, the CCI model rapidly transformed into a standardized technique . Adult male Sprague-Dawley rats were injured by a controlled cortical impact (CCI) delivered unilaterally to the parietal cortex, or were naïve controls. To the best of our knowledge, there is no closed-head model using a commercially available device, including the Controlled Cortical Impact (CCI) device. In the present study, we applied this technique to establish and characterize the CCI model of brain injury in another species, the mouse, evaluating cognitive and . The impact parameters vary considerably among different laboratories, making the comparison of research findings difficult. We have previously described CCI procedures in adult rats [18, 29] and reported its application to the immature rat [7, 11, 25, 33, 35, 64]. Together these features make CCI one of the most suitable experimental cerebral contusion models 26. Hypoxemia has detrimental effects after traumatic brain injury (TBI) in both experimental models and humans. Twenty-four normal cycling adult female rats underwent verification of estrous stage prior to controlled cortical impact (CCI) or sham injury and then were assigned to EE or standard (STD) housing. To test this hypothesis, a focal cortical contusion was induced using a controlled cortical impact (CCI) model in Sprague-Dawley rats. Key Words: Traumatic brain injury­ Magnetic resonance imaging-Perfusion-Rat-Controlled cortical impact. Controlled-cortical impact resulted in a large lesion of motor cortex. The effect of posttraumatic hypothermia (brain temperature controlled at 32°C for 4 h) on mortality after severe controlled cortical impact (CCI) was studied in rats. juvenile rats, but few have been developed in the adult rat. Methods: Anesthetized rats were randomized to receive right . The trauma protocol was approved by the Massachusetts General Hospital Institutional Animal Care and Use Committee and complied with the NIH Guide for the Care and Use of Laboratory Animals. We sought to determine sensitivity of the cortical impact injury model of traumatic brain injury (TBI) to severity of injury and to treatment. Nitric oxide (NO) and the NO end products, nitrate and nitrite, were measured at the impact site after a 5-m/s, 3-mm deformation controlled cortical impact injury in rats. Rats exposed to methylazoxymethanol acetate at embryonic Day 15 had a thinner cortical mantle compared with rats exposed at embryonic Day 17 and control animals. Our primary goal was to develop a set of CCI parameters that results in restricted damage to the CFA, sparing neurons in the RFA, but producing . This study systematically examined the effects of varying magnitudes of controlled cortical impact to the rat brain on neurological, cardiovascular, and histopathological variables. T1 - Reversal of attenuation of cerebrovascular reactivity to hypercapnia by a nitric oxide donor after controlled cortical impact in a rat model of traumatic brain injury. Twenty-four normal cycling adult female rats underwent verification of estrous stage prior to controlled cortical impact (CCI) or sham injury and then were assigned to EE or standard (STD) housing. (A) A representative drawing of the typical extent of damage caused by the controlled cortical impact. 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